韩国免费A级作爱片无码_亚洲欧洲精品成人久久av18_亚洲国产成人AV毛片大全_成年人在线免费看的惊悚动作片_一级爽片_爱爱免费

English | 中文版 | 手機版 企業登錄 | 個人登錄 | 郵件訂閱
當前位置 > 首頁 > 技術文章 > 非乙;疌AMP ELISA試劑盒使用說明書

非乙;疌AMP ELISA試劑盒使用說明書

瀏覽次數:808 發布日期:2023-1-4  來源:本站 僅供參考,謝絕轉載,否則責任自負
Product Highlight
  • Based on the unique mouse monoclonal anti-cAMP antibody
  • The only assay kit on the market with monoclonal anti-cAMP antibody (all other kits are based on polyclonal anti-cAMP antibodies)
  • The monoclonal anti-cAMP antibody displays much higher selectivity over cGMP and ATP than polyclonal anti-cAMP antibody
  • Avoids the batch-to-batch variations associated with polyclonal antibody productions from animals, thus providing the reproducibility
Product Description
Adenosine 3′, 5′-cyclic monophosphate (cyclic AMP; cAMP) modulates various physiological functions such as cardiovascular biology, learning and memory, olfaction, immune response, asthma and kidney function (1,2). cAMP is produced from ATP by adenylyl cyclases and is degraded by phosphodiesterases. Stimulation of adenylyl cyclases or inhibition of phosphodiesterases can increase cellular cAMP concentrations. Blockers of adenylyl cyclase-activating receptors and inhibitors of the cAMP-specific phosphodiesterases are used for treating human diseases. For example, blocking agents for cAMP-increasing beta-adrenergic receptors (beta-blockers) are used for treating abnormal heart rhythms, high blood pressure (hypertension), myocardial infarction and heart failure. Inhibitors of cAMP specific phosphodiesterase types 2 and 4 are being tested for cognition enhancement.

 
To screen for inhibitors or stimulators of cellular cAMP levels, it is essential to have a sensitive, selective and reproducible method to measure the cAMP concentrations. This is especially true for the initial screenings given the possible weaker effects of larger pools of compounds.
 
Currently available other ELISA kits measuring cAMP levels are based on the non-affinity-purified polyclonal anti-cAMP antibody. Despite the claimed selectivity, these polyclonal anti-cAMP antibodies display certain cross-reactivity with ATP. Given that ATP is the substrate for the cAMP production, it is very desirable to have an antibody with high specificity towards cAMP over ATP.
 
NewEast Biosciences cAMP ELISA kit is based on the unique mouse monoclonal anti-cAMP antibody. This monoclonal anti-cAMP antibody displays >108 fold of selectivity over ATP, cGMP, and other nucleoside analogues. NewEast Biosciences cAMP ELISA kit provides significantly improved sensitivity and selectivity over other kits based on polyclonal anti-cAMP antibodies. Our monoclonal anti-cAMP antibody-based ELISA kit also avoids the batch-to-batch variations associated with polyclonal antibody productions from animals, thus providing the reproducibility in the long run.
 
Principle Outline
NewEast Biosciences cAMP ELISA Kit is a competitive immunoassay to measure the cAMP levels, either from cell extracts or from in vitro adenylyl cyclase assays. Briefly, multi-well plates are coated with goat-anti-mouse serum. cAMP in cell extracts or in in vitro adenylyl cyclase assays will competitively bind to the monoclonal anti-cAMP antibody in the presence of fixed amounts of cAMP-conjugated horse-radish peroxidase or alkaline phosphatase. Known amounts of cAMP are used as standards to generate the calculation curve. After a short incubation, the excess reagents are washed away and substrate is added. The multiwell plates are then read on a microplate reader at 450 nm or 405 nm. The intensity of the yellow color is inversely proportional to the concentration of cAMP in samples. The measured optical density is used to calculate the concentration of cAMP in samples based on the curve from the cAMP standards.

 
發布者:武漢費斯德生物科技有限公司
聯系電話:027-63487286
E-mail:nebio0923@163.com

用戶名: 密碼: 匿名 快速注冊 忘記密碼
評論只代表網友觀點,不代表本站觀點。 請輸入驗證碼: 8795
Copyright(C) 1998-2025 生物器材網 電話:021-64166852;13621656896 E-mail:info@bio-equip.com
主站蜘蛛池模板: 亚洲一区二区三区AV天堂 | 亚洲干综合| 日韩一区二区观看 | 边摸边脱边吃奶边做视频 | 国产精品欧美久久久久久日本一道 | 日本免费看片 | 人妻日韩视频一区二区 | 久久夜色精品国产亚洲 | 好了av四色综合无码 | 免费观看无遮挡WWW的视频 | 欧美人妻日韩精品 | 亚洲成aⅴ人最新无码 | 天天躁狠狠躁夜躁2020挡不住 | 久在线观看视频 | 国产18禁黄网站免费观看 | 久久AV无码精品人妻出轨 | 啦啦啦视频在线观看高清免费 | 亚洲日韩AV无码中文 | 无码中文字幕乱码一区 | 亚洲国产成人久久精品软件 | GOGO亚洲肉体艺术 | 3ce奇米四色眼影奇米8888 | 国产露脸150部国语对白 | 精品AV无码国产一区二区 | 最近更新在线中文字幕 | 日本少妇特殊按摩2 | 日韩高清不卡一区二区 | 四虎在线播放 | 亚洲人成人伊人成综合网无码 | 国产一区二区3区 | 国产精品一区网站 | 国产CHINESEXXXXDIANJIANFEN | 国产在线一区二区综合免费视频 | 97人人超碰国产精品最新 | 中文国产| 无码专区久久综合久综合字幕 | 国产做A爰片毛片A片美国 | 国产精品成人无码久久久 | caoporn国产免费 | 久久精品国自产拍天天拍最新章节 | 精品亚洲国产成av人片传媒 |