Q. 鉑金版ELISA試劑盒中有哪些組分？

What do Platinum ELISA Kits contain?

A、 試劑盒中包含ELISA實(shí)驗(yàn)用的所有組分，同時(shí)包含優(yōu)化過的預(yù)包被酶標(biāo)板。All reagents necessary to perform 1 or 10 plate ELISA assays, including optimized capture-antibody pre-coated plates.

Q. 鉑金版ELISA試劑盒適用于檢測哪些樣本類型？

Which samples are suitable for Platinum ELISA Kits?

A、 大部分鉑金版ELISA試劑盒可以檢測常見ELISA樣本，比如：血清、血漿、細(xì)胞上清及其他體液樣本。詳細(xì)信息可以查看對應(yīng)產(chǎn)品的說明書。

Most ELISAs are suitable for serum, plasma, cell culture supernatants and other body fluids.Detailed information is given in the product manual for each individual kit.

Q. 使用鉑金版ELISA試劑盒，標(biāo)曲和樣本是否必須做復(fù)孔？

Do I have to run the Platinum ELISA standards and samples in duplicates?

A、 不是必須。但是為了結(jié)果準(zhǔn)確性，我們還是強(qiáng)烈建議您標(biāo)準(zhǔn)曲線和樣本做一次重復(fù)。No, but it is highly recommended to make at least double determinations of each sample and standard point.

Q. 不同試劑盒或者不同批次的鉑金版試劑盒中的組分能否混用？

Is it possible to use the reagents of other Platinum ELISA kits/lots?

A、 不可以。

The reagents within the Platinum ELISA kits are not interchangeable between different lots of the same kit or between kits against different analytes.

Q. 不同批次試劑盒說明書能參考使用嗎？

Can I always use the same manual for different ELISA kit lot numbers?

A、   不推薦。建議您參考隨貨收到的產(chǎn)品說明書。

We recommend that you use the manual included with the Platinum ELISA kit.

Q. 鉑金版ELISA試劑盒使用前有必要洗滌酶標(biāo)板嗎？

Is it necessary to wash the Platinum ELISA plates before usage?

A、     初始洗滌不是必須，但是為了檢測結(jié)果更加準(zhǔn)確，我們建議您進(jìn)行該步驟的操作。

An initial washing step is not necessary but recommended as accuracy may be increased.

Q. ELISA試劑盒說明書中的孵育時(shí)間可以隨意延長或者縮短嗎？

Are shorter or longer incubation times possible?

A、 為了獲得更準(zhǔn)確的檢測結(jié)果，強(qiáng)烈建議您按照產(chǎn)品說明書推薦的孵育時(shí)間進(jìn)行實(shí)驗(yàn)。 Incubation times should be followed exactly as stated in the product manual to ensure optimal Platinum ELISA test performance.

Q. 使用鉑金版ELISA檢測樣本時(shí)，我可以不稀釋自己的樣本，直接檢測嗎？ Can I omit the dilution of my Platinum ELISA samples with assay buffer/sample diluent when performing the assay?

A、 建議按照說明書推薦稀釋度操作。

We recommend following the instructions for sample dilutions as provided in the manual. We cannot guarantee optimal performance of the assay if internal assay dilutions as instructed were omitted.

Q. 如果檢測時(shí)發(fā)現(xiàn)試劑盒中組分看上去不足，怎么辦？What if it does not look like the supplied reagent volume is sufficient for running the Platinum ELISA?

A、 如果試劑看上去體積少，請務(wù)必在使用前短暫離心確保管內(nèi)液體被收集到管底。In case of small reagent volumes, please spin down vial before use to make sure to all contents are collected in the bottom of the tube.

Q. 除了試劑盒要求的保存方法，我可以按照其他方法保存嗎？

Is it possible to store the reagents other than indicated?

A、 不推薦。如果試劑盒不按照說明保存，那么無法保證鉑金版ELISA試劑盒質(zhì)量。 Performance of Platinum ELISA cannot be guaranteed if reagents are not stored as indicated.

Q. 鉑金版ELISA試劑盒中是否有針對正常樣本的檢測值？ Do you have information regarding the normal values detectable with your Platinum ELISA Kit?

A、 您可以在對應(yīng)產(chǎn)品說明書中“Expected Values”部分找到正常樣本期望值。 但是這些值是我們實(shí)驗(yàn)室檢測有限的樣本后得到數(shù)據(jù)。我們強(qiáng)烈建議您的實(shí)驗(yàn)室通過檢測您的研究群體獲得樣本正常值。 You can find expected "normal levels" listed in the Platinum ELISA Kit manual under "Expected Values".Please be aware that these normal values were obtained in our laboratories with a limited number of samples. We strongly suggest that each laboratory perform studies in order to establish normal range for their sample populations.

以上信息來源于eBioscience 官網(wǎng)：

http://us.ebioscience.com/resources/faq/elisa-faq.htm#Platinum-ELISA-Kits。

閱讀原文：http://www.liankebio.com/TechServiceShow/articleID/2014090002.html。