N6培養基被定義用來改善水稻花粉愈傷組織的形成、生長和分化。培養基中銨鹽的濃度對于愈傷組織的形成是至關重要的。NH4+離子濃度的最適范圍是7.0 mM（或3.5mM(NH4)2SO4)）。高濃度的銨鹽會顯著抑制水稻花粉的生長和分化。KNO3和培養基中其他成分的濃度不會影響花粉的生長。
Chu C.C, The N6 medium and its application to anther culture of cereal crops, Proc. Symp. Plant Tissue Cult., Peking, 43 (1978).
Chu C.C. et al., Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic., 18, 659 (1975).