韩国免费A级作爱片无码_亚洲欧洲精品成人久久av18_亚洲国产成人AV毛片大全_成年人在线免费看的惊悚动作片_一级爽片_爱爱免费

English | 中文版 | 手機版 企業登錄 | 個人登錄 | 郵件訂閱
當前位置 > 首頁 > 技術文章 > Trabecular cell monolayer culture

Trabecular cell monolayer culture

瀏覽次數:2460 發布日期:2011-9-2  來源:www.pricells.com.cn
                                Trabecular cell monolayer culture   
Originally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for investigation of the molecular and biochemical functions in this distinct cell type.  The trabecular meshwork accounts for most aqueous outflow resistance in the anterior chamber of the human eye.1 It is believed that the interplay between the endothelial-like cells of this tissue (trabecular cells) and the surrounding extracellular matrix are responsible for maintaining the resistance necessary for preservation of the aqueous outflow pathway.  The trabecular cells are phagocytic cells, actively removing debris such as pigment granules, erythrocytes, and pseudoexfoliation material from the aqueous outflow system.  They also are involved in the production and turnover of extracellular matrix.

1. The trabecular meshwork was dissected from human donor eyes and subjected to extracellular matrix digestion with solution of primary cell isolation.

2. The cells were pelleted and resuspended in primary cell culture system containing penicillin-streptomycin (100 U/mL) and 10% FBS (DMEM-FBS).

3. Cells were placed in a T25 flask and grown to confluence at 37°C in 5% CO2 in primary cell culture system (passages 3–7).

4. The confluence of cell cultures was determined by observation of cell density at 100× magnification with an inverted light microscope.

5. Cultures were considered confluent when cell expansion had reached a point where cells touched each other on all sides and no intercellular gaps were present.

6. Once cells appeared confluent, trabecular cells were maintained in primary cell culture system for an additional 3 to 7 days to ensure a confluent state.

7. After incubation in primary cell culture system, cells were washed twice with phosphate-buffered saline (PBS) and grown in primary cell culture system and 50% human aqueous for up to 21 days.

Morphologic appearance of primary trabecular cell cultures. Trabecular cells incubated for 21 days.  Cells displayed a broad, flat appearance when compared with the spindle shape of cells grown. Magnification, ×200.

Reference
1. Michael P. Fautsch, Kyle G. Howell, Anne M. Vrabel, M. Cristine Charlesworth, David C. Muddiman and Douglas H. Johnson. Primary Trabecular Meshwork Cells Incubated in Human Aqueous Humor Differ from Cells Incubated in Serum Supplements. Invest. Ophthalmol. Vis. Sci. 2005: 46: 2848-2856.

2. Polansky JR, Weinreb RN, Baxter JD, Alvarado J. Human trabecular cells. I. Establishment in tissue culture and growth characteristics. Invest Ophthalmol Vis Sci. 1979; 18: 1043–1049.

3. Stamer WD, Seftor REB, Williams SK, Samaha HAM, Snyder RW. Isolation and culture of human trabecular meshwork cells by extracellular matrix digestion. Curr Eye Res. 1995;14:611–617.

發布者:武漢原生原代生物醫藥科技有限公司
聯系電話:027-87490190
E-mail:service@pricells.com.cn

用戶名: 密碼: 匿名 快速注冊 忘記密碼
評論只代表網友觀點,不代表本站觀點。 請輸入驗證碼: 8795
Copyright(C) 1998-2025 生物器材網 電話:021-64166852;13621656896 E-mail:info@bio-equip.com
主站蜘蛛池模板: 亚洲女优在线播放 | 国产成人无码A区在线观看视频 | 精品国产一区二区三区无码 | 国产精品videossexohd | 国产成人专区 | 婷婷亚洲综合五月天小说 | 色aaaa | 国产精品揄拍一区二区 | 99国内精品 | 熟女内射v888av | 日韩精品 在线 国产 丝袜 | 最新理论三级中文在线观看 | 无码少妇一区二区性色Av | 在线免费看av不卡 | 国产伊人av| 国产精品久久久久久久第一福利 | 丰满人妻一区二区三区av猛交 | 国产真实乱在线更新 | 精品日韩欧美一区二区三区 | 欧美a级情欲片 | 国产自产视频一区二区三区 | 亚洲国产福利成人一区二区 | 给我免费观看片在线观看中国 | 午夜无码片在线观看影院Y 伊人小视频 | 国产国拍精品亚洲A片男同 亚洲av日韩av天堂影片精品 | 人人澡人人人人天天夜夜 | 国产精品久久久久久久久免费相片 | 精品人妻中文av一区二区三区 | 亚洲高清偷拍一区二区三区 | 一级黄色毛片免费观看 | 久热在线中文字幕色999舞 | 日本高清中文字幕 | 农村妇女野战bbxxx | 国产亚洲毛片 | 91网站免费在线?看 欧洲成年人性生活免费视频 | 国产成人精品无码免费视频 | 国产亚洲h网综合h网 | 嫩草在线看 | 啊灬啊灬啊灬快灬深用力A片 | 日本不卡一二三区 | 国产成人综合欧美午夜不卡 |